Solid phase enzyme-linked competitive binding assay for riboflavin

Solid phase enzyme-linked competitive binding assay for riboflavin.

A new solid-phase enzyme-linked assay for riboflavin (vitamin B2) is described. The assay is based on the competition between analyte vitamin molecules and a glucose-6-phosphate dehydrogenase-3-carboxymethylriboflavin conjugate for a limited number of riboflavin-binding protein sites immobilized on Sepharose particles. Significant improvements in conjugate catalytic activity and thus detectabil...

H9mogeneous Enzyme-linked Competitive Binding Assay for Riboflavin

A rapid and precise homogeneous enzyme-linked competitive binding assay for riboflavin (vitamin Bz) is described. The method utilizes a malate dehydrogenase/3-carboxymethylriboflavin conjugate in conjunction with soluble riboflavin binding protein. In the absence of the vitamin, the catalytic activity of the enzyme/riboflavin conjugate is inhibited up to 71% by the binding protein. In the prese...

Enzyme-Linked Immunosorbent Assay Competitive Binding Inhibition

A Solid-Phase Enzyme-Linked Assay for Vitamin B12

A new solid-phase enzyme-linked competitive binding assay for vitamin B12 (cyanocobalamin) is described. The assay is based on the competition between analyte B12 molecules and a glucose-6-phosphate dehydrogenase-vitamin B12 conjugate for a limited number of R-protein binding sites immobilized on sepharose particles. After appropriate incubation and washing steps, the enzyme activity bound to t...

Homogeneous Enzyme-linked Competitive Binding Assay for Biotin Based on the Avidin-biotin Interaction

A homogeneous enzyme-linked competitive-binding assay for biotin with glucose-6-phosphate dehydrogenase (GGPDH), is described. This assay is based on the interaction between a GGPDH/biotin conjugate with avidin, a natural binder for biotin. In the absence of biotin in the assay mixture, this interaction results in 100% inhibition of the enzyme conjugate. In the presence of biotin, the enzymatic...